(Universitas Pembangunan Nasional "Veteran" Jawa Timur, Indonesia)(2) Tri Mujoko
(UPN “Veteran†Jawa Timur, Indonesia)(3) * Arika Purnawati
(UPN “Veteran†Jawa Timur, Indonesia)*corresponding author
AbstractBercak bakteri merupakan salah satu penyakit pada tanaman tomat yang disebabkan oleh bakteri patogen Xanthomonas campestris pv. vesicatoria. Metabolit sekunder Trichoderma harzianum memiliki kemampuan sebagai antibakteri, sehingga berpotensi untuk mengendalikan bakteri Xanthomonas campestris pv. vesicatoria. Penelitian ini bertujuan untuk mengetahui kemampuan konsentrasi metabolit sekunder T. harzianum yang berpotensi dalam menghambat pertumbuhan bakteri Xanthomonas campestris pv. vesicatoria. Metode penelitian menggunakan rancangan acak lengkap dengan 5 perlakuan yaitu konsentrasi 0%, 25%, 50%, 75%, 100% dan diulang 4 ulangan. Pelaksanaan penelitian ini yaitu isolasi bakteri Xanthomonas campestris pv. vesicatoria, peremajaan jamur T. harzianum, produksi metabolit jamur T. harzianum, ekstraksi metabolit sekunder dan uji antagonis in vitro. Parameter yang diamati adalah luas zona hambatan dan karakteristik bakteri Xanthomonas campestris pv. vesicatoria. Hasil penelitian menunjukan semua konsetrasi dapat menekan pertumbuhan bakteri Xanthomonas campestris pv. vesicatoria dengan konsentrasi 100% menghasilkan zona hambat tertinggi yaitu sebesar 16 mm.
KeywordsMetabolit sekunder, Trichoderma harzianum, Xanthomonas campestris pv. vesicatoria
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DOIhttps://doi.org/10.31604/jap.v7i3.7046 |
Article metrics10.31604/jap.v7i3.7046 Abstract views : 0 | PDF views : 0 |
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